Title

Effect of TLCK on transcription and its role in modifying cell-growth.

SelectedWorks Author Profiles:

Norine E. Noonan

Document Type

Article

Publication Date

1977

Date Issued

1977-01-01

Date Available

2013-06-12

ISSN

0021-9541

Abstract

The synthetic protease inhibitor N-tosyl-L-lysine-chloromethyl ketone (TLCK) acts to inhibit transcription when added to cell lines growing in vitro. This inhibition of transcription is most pronounced in transformed cells where TLCK is very toxic at concentrations as low as 25 mug/ml of culture medium. Non-transformed cells are more resistant to the effect of TLCK, requiring ten times more TLCK to produce a comparable inhibition of transcription. The effect of this protease inhibitor on transcription can be prevented by preincubation of the cells in reduced glutathione or cysteine; however, the cells can not be rescued from the effect of TLCK even if glutathione or cysteine are added to the culture medium within five minutes of the addition of TLCK.

Comments

Abstract only. Full-text article is available only through licensed access provided by the publisher. Published in Journal of Cellular Physiology, 92(1), 137-143. DOI: 10.1002/jcp.1040920116

Language

en_US

Publisher

John Wiley & Sons, Inc.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.